Active Projects

Perturbed cell metabolism and energy regulation is a well established hallmark of cancer and has been studied since the middle of the nineteenth century. Cancer cells have been shown to exhibit greater activity of metabolic pathways including the glycolytic and glutaminolysis pathways and a reduced dependence on oxidative phosphorylation. However the mechanism by which cancer cells switch from traditional metabolic programs to their characteristic alternate programs remains poorly understood.

Such reprogramming events involve dynamic regulation of many genes and gene networks. My hypothesis is that these regulatory events are mediated through epigenetic means including chromatin modifications and functional long-noncoding RNAs. I am testing this hypothesis in the context of T-cell acute leukemia.

Protocol Bank

Here you can find links to some of my (mostly wet-lab) protocols.

Chromatin Immunoprecipitation (ChIP)

This protocol is my standard ChIP protocol which is currently optimized for histone/histone modification ChIP-seq. For ChIP of transcription factors or TF co-factors, one should optimize the sonication conditions (or perhaps use MNase digest instead) and crosslinking steps. Download PDF

Immunoflorescence Staining (IF)

This protocol is my standard IF staining protocol. This version of the IF protocol describes IF starting with cells growing in culture. Fixation and permeabilization steps require selection/optimization based on the location of the target protein. 3-Days total time. Download PDF

“Satisfaction of one's curiosity is one of the greatest sources of happiness in life.

Linus Pauling